Processes Of Recombinant DNA Technology Test

Processes Of Recombinant DNA Technology

This is online test 01 for CBSE class 12 Biology Biotechnology : Principles and Processes (Processes of Recombinant DNA Technology).. There are 10 questions in this test with each question having around four answer choices.

Questions & Answers

1
The first step of recombinant DNA technology involves
  • A
    Insertion of recombinant DNA in host cell
  • B
    Amplification of GOI
  • C
    Cutting of DNA at specific location
  • D
    Isolation of genetic material
    Correct
2
Overhangs produced by restriction enzymes are called as
  • A
    Palindromic sequences
  • B
    Sticky ends
    Correct
  • C
    Polymers
  • D
    Blunt ends
3
E. coli carry resistance to
  • A
    Fluoroquinolone
    Correct
  • B
    Ampicillin
  • C
    chloramphenicol
  • D
    Tetracycline
4
Primers are
  • A
    Palindromic sequences
  • B
    Chemically synthesized oligonucleotides
    Correct
  • C
    Nucleases
  • D
    Fragments of RNA
5
The separation and purification of recombinant protein product is called as
  • A
    Downstream processing
    Correct
  • B
    Extraction
  • C
    Tissue culture
  • D
    Hybridisation
6
The smallest bands in the agarose gel will be towards
  • A
    Anode
    Correct
  • B
    Cathode
  • C
    Middle of the gel
  • D
    Wells
7
The property of Taq polymerase explored in PCR is
  • A
    Exonuclease activity
  • B
    Thermolabile nature
  • C
    High fidelity
  • D
    Resistance to high temperature
    Correct
8
Electroporation helps in the introduction of foreign DNA into target organism by
  • A
    Gold coated particles
  • B
    Microinjections
  • C
    Increasing electrical permeability of the membrane
    Correct
  • D
    Helium gas
9
Helium is used in Biolistic gun as
  • A
    Tungsten coated with DNA can be easily passed in the presence of Helium
  • B
    It acts as a propellant
    Correct
  • C
    It is a light gas
  • D
    It is an inert gas
10
Thermostable DNA polymerase is isolated from bacterium
  • A
    Escherichia coli
  • B
    Salmonella typhii
  • C
    Rhizobium
  • D
    Thermus aquaticus
    Correct