Tools Of Recombinant DNA Technology Test

Tools Of Recombinant DNA Technology

This is online test 01 for CBSE class 12 Biology Biotechnology : Principles and Processes (Tools of Recombinant DNA Technology).. There are 10 questions in this test with each question having around four answer choices.

Questions & Answers

1
In Genetic Engineering, to cut DNA at a specific site, the enzyme used is
  • A
    RNA polymerase
  • B
    Restriction enzyme
    Correct
  • C
    ß- galactosidase
  • D
    DNA polymerase
2
The enzyme ligase is used to
  • A
    Cut the vector DNA
  • B
    Cut goi from the source DNA
  • C
    Breakdown of lipids to fatty acids and glycerol
  • D
    Join vector and goi (gene of interest)
    Correct
3
Restriction enzymes belongs to a larger class of enzymes called
  • A
    Chitinase
  • B
    Protease
  • C
    Nucleases
    Correct
  • D
    Glcucatase
4
The first restriction endonuclease discovered was
  • A
    PuvI
  • B
    Hind II
    Correct
  • C
    EcoRI
  • D
    HindIII
5
The extraction of DNA from the agrose gel is called as
  • A
    Ligation
  • B
    Elution
    Correct
  • C
    Isolation
  • D
    Transformation
6
Proteins can be digested by
  • A
    Protease
    Correct
  • B
    Nuclease
  • C
    Pectinase
  • D
    Chitinase
7
DNA is extracted by
  • A
    Heat shock
  • B
    Denaturation
  • C
    Chilling treatment
  • D
    Ethanol Precipitation
    Correct
8
The nuclease will act on
  • A
    DNA only
  • B
    RNA only
  • C
    DNA and RNA
    Correct
  • D
    Proteins
9
Eco RI acting on goi and vector will produce
  • A
    Blunt ends in both
  • B
    Blunt and sticky ends respectively
  • C
    Sticky ends in both
    Correct
  • D
    Sticky and blunt ends respectively
10
The polyacrylamide gel is used to separate proteins as
  • A
    Proteins are big as compared to DNA
  • B
    Proteins cannot be run in agarose gel
  • C
    Agarose gel reacts with protein
  • D
    Proteins are small as compared to DNA
    Correct