Processes Of Recombinant DNA Technology Test

Processes Of Recombinant DNA Technology

This is online test 03 for CBSE class 12 Biology Biotechnology : Principles and Processes (Processes of Recombinant DNA Technology).. There are 10 questions in this test with each question having around four answer choices.

Questions & Answers

1
Sparged stirred tank bioreactor is advantageous over the simple stirred tank bioreactor as
  • A
    Sparged tank has blades which move fast
  • B
    Vitamins and minerals are more used up in sparged stirred bioreactor
  • C
    It requires less of maintenance
  • D
    Air bubbles enhance the oxygen transfer area
    Correct
2
The X-gal will be converted into a coloured product when
  • A
    When lactose is available
  • B
    Gene coding for B- galactosidase is cleaved
  • C
    When goi is inserted in the vector at the site coding for B- galactosidase
  • D
    B–galactosidase acts on it
    Correct
3
Blades in a bioreactor help in
  • A
    mixing of all the components and prevent their settling
    Correct
  • B
    provides air bubbles
  • C
    provides ample space for the formation of recombinant protein product
  • D
    provides energy to bacterial cells
4
The restriction sites of the nucleoid are methylated because
  • A
    to protect bacteria from its own enzymes
    Correct
  • B
    methylation helps in easy cutting by the molecular scissors
  • C
    methylation helps in easy transfer of DNA
  • D
    methylation is important for the identification by restriction enzymes
5
A ladder is used in Gel electrophoresis as it helps in
  • A
    it helps in denaturation of DNA
  • B
    it helps in EtBr binding
  • C
    It prevents the movement of DNA out of the gel
  • D
    Comparing the size of the DNA fragment
    Correct
6
What modification in the Ti plasmid of Agrobacterium tumifaciens is done to use it as a cloning vector which is not pathogenic
  • A
    Ti genes are removed
    Correct
  • B
    Antibiotic resistant gene is introduced
  • C
    Promoter is removed
  • D
    rop genes are removed
7
If a goi is inserted at the Bam HI site in Pbr322, the plasmid
  • A
    Will lose ampicillin resistance
  • B
    Will lose both ampicillin and tetracycline resistance
  • C
    Will lose tetracycline resistance
    Correct
  • D
    Will not replicate
8
The agarose gel with high % of agar will lead to accumulation of large sized DNA fragment near the wells as
  • A
    Voltage fluctuation with high % of agar
  • B
    Pore size is big
  • C
    Absence of pores
  • D
    Pore size is small
    Correct
9
The sequence of the 5’ and 3’ primers for the following DNA sequence will be 5’ AATGCGGCAATCGAGTC3’ 3’ TTACGCCGTTAGCTCCAG5’
  • A
    5’ AATGC 3’, 5’ TTACG 3’
  • B
    5’ AATGC 3’, 5’ GACTC 3’
    Correct
  • C
    5’ TTACG 3’, 5’ CTGAG 3’
  • D
    5’ AGTC 3’ , 5’ TCAG 3’
10
Which of the following is correct sequence of process of recombinant DNA technology- i. Isolation of DNA ii. Isolation of desired DNA fragment. iii. Fragmentation of DNA by restriction endonuclease. iv. Transferring of into host v. Ligation of DNA fragment into vector vi. Culturing in host cell to get desired product.
  • A
    Step i, iii, ii, v, iv and vi
    Correct
  • B
    Step i, iv, iii, ii, v and vi
  • C
    Step v, vi, iv, iii, ii and i
  • D
    Step i, ii, iii, iv, v and vi