Principles Of Biotechnology Test

Principles Of Biotechnology

This is online test 03 for CBSE class 12 Biology Biotechnology : Principles and Processes (Principles of Biotechnology).. There are 10 questions in this test with each question having around four answer choices.

Questions & Answers

1
Tracking dye should be used in a gel as to
  • A
    To prevent EtBr from interacting with the DNA fragments
  • B
    Track the position of DNA in the gel
    Correct
  • C
    To track the voltage fluctuation
  • D
    To prevent the flow of EtBr in the gel tank
2
The tracking dye BPB can be used because it has high density so
  • A
    It leads to the sinking of DNA samples in the well
    Correct
  • B
    It helps in staining with Etbr
  • C
    It leads to floating of DNA samples out of the well
  • D
    Absence of glycerol
3
Which one is called molecular scissors?
  • A
    Exonuclease
  • B
    Ribonuclease
  • C
    Endonuclease
  • D
    Restriction enzymes
    Correct
4
Agrobacterium tumifaciens is called as the natural genetic engineer as
  • A
    It can be disarmed
  • B
    It is a plant pathogen
  • C
    It causes crown gall
  • D
    Gene transfer occurs without any human effort
    Correct
5
Denaturation is done at high temperature as it leads to
  • A
    Polymerization
  • B
    Termination of the reaction
  • C
    Breaking of hydrogen bonds between the bases
    Correct
  • D
    Joining of primers at the 5’ end
6
The technique of genetic engineering includes creation of recombinant DNA, use of gene cloning and
  • A
    Gene transfer
    Correct
  • B
    Gene mutation
  • C
    All of the above
  • D
    Gene polymerization
7
Steps involved in genetic engineering are: Maintenance of introduced DNA in the host Transfer of DNA in its progeny Identifying DNA with desirable genes Introduction of identified DNA into the host
  • A
    2, 3, 1, 4
  • B
    1, 2, 3, 4
  • C
    3, 4, 1, 2
    Correct
  • D
    4, 3, 2, 1
8
If a DNA is fragmented using Eco RI , the fragments that will be produced are
  • A
    5’ ATG GAC3’ 3’ TAC CT G5’
  • B
    5’ GAA TCC3’ 3’CAG CT G5’
  • C
    5’ G AATTC3’ 3’CTTAA G5’
    Correct
  • D
    5’ GG TT3’ 3’CC AA5’
9
Technique of genetic engineering includes creation of
  • A
    Recombinant DNA, gene cloning and gene transfer.
    Correct
  • B
    Creation of recombinant DNA only
  • C
    Gene transfer only
  • D
    Gene cloning and gene transfer
10
Origin of replication is the specific DNA sequence on chromosome that which is responsible for
  • A
    Synthesis of polymerase enzyme
  • B
    All of the these
  • C
    Terminating replication
  • D
    Initiating replication
    Correct