Tools Of Recombinant DNA Technology Test

Tools Of Recombinant DNA Technology

This is online test 02 for CBSE class 12 Biology Biotechnology : Principles and Processes (Tools of Recombinant DNA Technology).. There are 10 questions in this test with each question having around four answer choices.

Questions & Answers

1
The group of letters that form same words when read both forward and backward are called
  • A
    Palindrome
    Correct
  • B
    Puzzle
  • C
    Sticky ends
  • D
    Endonucleases
2
Enzymes used to join foreign DNA to plasmid is
  • A
    Ligases
    Correct
  • B
    Endonucleases
  • C
    Nucleases
  • D
    Pectinases
3
The machine used to do PCR is called as
  • A
    Thermocycler
    Correct
  • B
    Voltage Regulator
  • C
    Temperature regulator
  • D
    Heater
4
Molecular scissors is the name given to
  • A
    Polymerase
  • B
    Phosphatase
  • C
    Ligase
  • D
    Restriction enzymes
    Correct
5
DNA is amplified by PCR as it can be used for
  • A
    It requires dNTP’s
  • B
    To produce multiple copies of the fragment DNA
    Correct
  • C
    It requires primers
  • D
    It requires \(M{g^{2 + }}\)
6
Agarose gel is used for electrophoresis because
  • A
    It is easily extractable
  • B
    It is inert
    Correct
  • C
    It is a polysaccharide
  • D
    It is cheap
7
If DNA is digested by EcoRI, it will lead to
  • A
    Sticky ends
    Correct
  • B
    Multiple ori
  • C
    No antibiotic resistance
  • D
    Blunt ends
8
Restriction endonucleases are used in genetic engineering to form
  • A
    All of the above
  • B
    Amplification of DNA fragment
  • C
    Joining two DNA segments
  • D
    Recombinant molecules of DNA
    Correct
9
‘R’ in EcoRI restriction endonuclease denotes
  • A
    Strain RY13
    Correct
  • B
    Sequence of isolation of the enzyme
  • C
    Genus
  • D
    Species
10
Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. Reason: we can see bright red coloured bands of DNA in aethidium bromide stained gel exposed to UV light.
  • A
    Assertion is correct but reason is incorrect.
    Correct
  • B
    Assertion is correct but reason does not explain the assertin.
  • C
    Both assertion and reason are incorrect.
  • D
    Both assertion and reason are correct.